Table 2 Primers used for qPCR
Gene target | GenBank accession | Direction | Primer DNA sequence | Size of the amplicon (pb) | RSq | PCR Efficiency (%) |
|---|---|---|---|---|---|---|
Hsp90 | isotig08469 | Fw | CCGATGCTTTGGACAAGATTC | 151 | 0.989 | 122.4 |
Rv | ATGTCAGCCTTGGTCATTCC | |||||
Hsc70 | isotig04537 | Fw | ACAAGAGGGCAGTCAGGAGA | 218 | 0.982 | 121.6 |
Rv | TTTGCCCAACTTGGAGTCAC | |||||
Hyou1 | isotig05161 | Fw | ATGACATGGGTGCTGGTAGT | 170 | 0.992 | 123.4 |
Rv | TGCAAGGTGCTTCTGAAGAC | |||||
Grp78 | isotig04367 | Fw | CGACTTGGGCACAACCTATT | 203 | 0.980 | 116.5 |
Rv | TTCCGATCAACCTCTTGGCAT | |||||
Gapdh | Fw | AAGGAGGAGCCAAGAAGGTC | 93 | 0.999 | 109.5 | |
Rv | TCCCTGCATCGTATTCCTTC | |||||
18S | Fw | GAGCCTGCGCTTAATTTGAC | 186 | 0.998 | 109.2 | |
Rv | GGCGCAACTATTTAGCAAGC |