|
Gene target
|
GenBank accession
|
Direction
|
Primer DNA sequence
|
Size of the amplicon (pb)
|
RSq
|
PCR Efficiency (%)
|
|---|
|
Hsp90
|
isotig08469
|
Fw
|
CCGATGCTTTGGACAAGATTC
|
151
|
0.989
|
122.4
|
|
Rv
|
ATGTCAGCCTTGGTCATTCC
|
|
Hsc70
|
isotig04537
|
Fw
|
ACAAGAGGGCAGTCAGGAGA
|
218
|
0.982
|
121.6
|
|
Rv
|
TTTGCCCAACTTGGAGTCAC
|
|
Hyou1
|
isotig05161
|
Fw
|
ATGACATGGGTGCTGGTAGT
|
170
|
0.992
|
123.4
|
|
Rv
|
TGCAAGGTGCTTCTGAAGAC
|
|
Grp78
|
isotig04367
|
Fw
|
CGACTTGGGCACAACCTATT
|
203
|
0.980
|
116.5
|
|
Rv
|
TTCCGATCAACCTCTTGGCAT
|
|
Gapdh
| |
Fw
|
AAGGAGGAGCCAAGAAGGTC
|
93
|
0.999
|
109.5
|
|
Rv
|
TCCCTGCATCGTATTCCTTC
|
|
18S
| |
Fw
|
GAGCCTGCGCTTAATTTGAC
|
186
|
0.998
|
109.2
|
|
Rv
|
GGCGCAACTATTTAGCAAGC
|
